Magnetic Bead Separators for Process Development and Scale-Up
Sepmag A200ml and A400ml are designed to develop, optimize and scale magnetic bead separation before GMP manufacturing.
Work from standard laboratory tubes to 100–400 mL process volumes, measure separation performance in real time and establish a reproducible process that can be transferred to larger-scale production.
When Do You Need Sepmag A?
Choose Sepmag A when your magnetic bead workflow is moving beyond small-volume research and you need to turn a laboratory protocol into a controlled, scalable process.
Sepmag A is designed for:
Process development
Protocol optimization
Scale-up studies
Method transfer
Pilot production
Validation studies
Troubleshooting before manufacturing
Preparation for GMP scale-up
It provides the intermediate step between small-volume Research & Analytical systems and large-volume Sepmag Q/N manufacturing systems.
Choose the Right Sepmag A System
Select the model according to your working volume and process vessel.
Both systems can also use adaptors for standard laboratory tubes.
This allows protocols to be developed or troubleshot at small volume before moving to 100–400 mL process volumes.
What You Gain During Process Development
Scale with Less Trial and Error
Moving to a larger vessel changes the magnetic separation step.
Sepmag A allows you to develop the process under controlled conditions and evaluate separation performance before transferring it to manufacturing.
This reduces uncertainty when moving from laboratory protocols to larger batch volumes.
Define the Correct Separation Endpoint
There is no universal magnetic separation time.
Real-time monitoring shows the complete separation curve and allows you to determine when separation is actually complete instead of relying only on visual inspection or an arbitrary waiting time.
Optimize the Process with Data
Compare separation curves while changing:
- Magnetic bead concentration
- Bead type or bead lot
- Buffer composition
- Sample viscosity
- Process volume
- Protocol conditions
This makes protocol optimization measurable rather than subjective.
Protect Recovery During Scale-Up
A process that works at a few milliliters does not automatically deliver the same recovery at hundreds of milliliters.
Controlled separation conditions help minimize bead and target loss as the process moves to larger volumes.
Improve Reproducibility
Establish defined separation conditions that can be repeated across runs, operators and development stages.
Real-time separation data also makes it easier to identify when a run behaves differently from the expected process.
Build a Transferable Process
Characterize the separation step before it reaches manufacturing.
Defined operating conditions, separation curves and objective endpoints provide a stronger basis for process transfer, validation and subsequent GMP production.
Measure Every Separation with Sepmag MONITOR
Sepmag MONITOR provides real-time optical measurement of magnetic bead separation with Sepmag A200ml and A400ml systems.
Instead of recording only how long the sample remained in the separator, you can measure how the beads actually separated.
Use separation curves to:
- Characterize separation kinetics
- Define an objective endpoint
- Compare protocols
- Compare bead lots
- Evaluate changes in process conditions
- Detect abnormal separation behavior
- Document process development
- Establish a reference profile for scale-up
From Laboratory Protocol to Manufacturing
Sepmag A is designed as the process-development bridge between research and GMP manufacturing.
Research & assay development
LITE / LAB / CELL 0.5–35 mL
Process development & scale-up
Sepmag A200ml / A400ml 100–400 mL
GMP manufacturing
Sepmag Q / N Liter-scale production
Developing the separation step under controlled and measurable conditions makes subsequent process transfer more systematic and reduces the amount of redevelopment required as volume increases.
Applications
Sepmag A can be used to develop and scale magnetic bead separation processes for:
DNA and RNA purification
Protein and antibody purification
Cell isolation
Diagnostic assay and reagent production
Magnetic bead manufacturing
Other magnetic bead-based bioprocesses
The system is selected primarily according to process stage and working volume, rather than a specific magnetic bead application.
Which Sepmag A System Do I Need?
Choose Sepmag A200ml for working volumes between 100 and 200 mL.
Choose Sepmag A400ml for working volumes between 200 and 400 mL.
Both systems support standard laboratory tubes using adaptors, allowing development work to be performed at smaller volumes when required.
If your process is moving into liter-scale GMP production, consider Sepmag Q for glass vessels or Sepmag N for plastic and single-use vessels.
Clients
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Frequently Asked Questions
When should I move from a small-volume separator to Sepmag A?
Use Sepmag A when you need to move beyond laboratory-scale separation and develop, optimize or characterize the process before manufacturing. Standard Sepmag A bottle configurations cover working volumes from 100 to 400 mL.
What is the difference between Sepmag A200ml and A400ml?
Sepmag A200ml is designed for 100–200 mL working volumes in 250 mL bottles. Sepmag A400ml is designed for 200–400 mL working volumes in 500 mL bottles.
Can Sepmag A be used with laboratory tubes?
Yes. Adaptors allow A200ml and A400ml systems to work with standard 1.5, 2, 15 and 50 mL tubes, making it possible to perform development and troubleshooting at smaller volumes.
How does Sepmag A support scale-up to manufacturing?
Sepmag A allows the magnetic separation step to be characterized using controlled separation conditions, real-time separation curves and objective endpoints. These process parameters provide a stronger basis for transfer to larger Sepmag Q or N manufacturing systems.
Develop a Magnetic Separation Process Ready to Scale
Tell us your:
Current volume → target volume → vessel → magnetic beads → application → manufacturing requirements
We will help you define the appropriate Sepmag A configuration and scale-up path.
