Real-Time Monitoring of
Magnetic Bead Separation
A clear sample only shows how the process ended. It does not show how the beads behaved during separation.
Sepmag MONITOR and QUALITANCE track magnetic bead separation in real time, generating objective process data instead of relying only on visual judgment or a predefined waiting time.
Define the separation endpoint, compare every run, detect deviations and document process performance.
Turn Magnetic Separation into Measurable Data
Define the Separation Endpoint
Determine when separation has reached the required endpoint instead of applying the same fixed time to every sample.
Compare Runs Objectively
Compare protocols, bead lots, batches, operators and process scales using quantitative separation data.
Detect Deviations Earlier
Identify unusual separation behavior before it affects recovery, consistency or downstream results.
Optimize Protocols Faster
Measure how changes in beads, buffers, concentration, viscosity or operating conditions affect separation performance.
Document Process Performance
Generate records for process standardization, technology transfer, validation and quality activities.
What Visual Inspection Cannot Show
Two samples may both look clear at the end of separation but may have behaved very differently.
One may have separated more slowly than expected. Another may have separated unusually quickly because of poor resuspension or bead aggregation. A predefined waiting time or visual endpoint cannot reveal these differences.
Real-time monitoring captures the entire process, not only its final appearance.
How Sepmag Monitoring Works
Measure Changes in Suspension Opacity
Optical sensors continuously track the reduction in opacity as magnetic beads move from the suspension to the retention area.
Generate the Separation Curve
The software plots the optical signal over time, showing how the separation progresses from the initial suspension to the final endpoint.
Quantify Process Performance
Parameters such as separation time, curve shape and intermediate or final endpoints provide objective information about bead behavior.
Compare with a Reference
Current results can be compared with previous runs or an approved reference profile to identify changes and confirm process consistency.
What the Separation Curve Tells You
When Separation Is Complete
Define an objective endpoint and avoid removing the supernatant before bead capture is sufficiently complete.
How Fast the Process Is Progressing
Quantify separation kinetics and determine whether the process is behaving as expected.
Whether Bead Behavior Has Changed
Variations in bead properties, concentration, resuspension, buffer viscosity or temperature can produce measurable changes in the curve.
Whether Runs Are Reproducible
Overlay or compare curves to assess consistency between experiments, bead lots, batches, operators or production sites.
Why Constant Magnetic Force Matters
Reliable monitoring requires well-defined magnetic conditions.
Sepmag applies a constant magnetic force throughout the working volume. Because bead movement is not affected by different magnetic-force zones within the vessel, changes in the separation curve are easier to relate to the bead suspension and process conditions.
This makes the data more useful for protocol development, batch comparison, troubleshooting and scale-up.
Monitoring from R&D to Manufacturing
MONITOR for Research and Process Development
Use MONITOR to:
- Characterize separation kinetics
- Compare experimental conditions
- Evaluate magnetic bead lots
- Optimize and standardize protocols
- Generate transferable process data
Objective Measurement
Use QUALITANCE to:
- Compare actual performance with a defined reference
- Monitor batch-to-batch consistency
- Identify process deviations
- Maintain traceable separation records
- Support validation and QA/QC activities
QUALITANCE data can be managed within Sepmag’s 21 CFR Part 11-ready and EUR GMP Annex 11 aligned software environment for regulated manufacturing workflows.
Stop Treating Separation as a Blind Step
Tell us your bead type, working volume and current separation method. Sepmag will help you determine how real-time monitoring can improve process understanding, reproducibility and scale-up.
