Constant Magnetic Force for
Reproducible Magnetic Bead Separation
Magnetic beads should not experience different separation conditions simply because of where they are located in the vessel.
Conventional magnetic separators create strong-force zones close to the magnet and weak-force zones farther away. Sepmag systems apply a constant, well-defined magnetic force throughout the working volume, giving magnetic beads controlled and predictable separation conditions.
The result: more complete bead recovery, less clumping, easier resuspension, more reproducible runs and lower scale-up risk.
What Constant Magnetic Force Changes
Complete Bead Recovery
Reduce the risk of beads remaining in suspension in low-force areas.
Less Clumping. Easier Resuspension.
Avoid excessive local force that can compress beads close to the magnet and make them difficult to redisperse.
Reproducible Runs
Make bead movement and capture less dependent on starting position, operator judgment or minor process variations.
Lower Scale-Up Risk
Transfer defined magnetic separation conditions across volumes instead of redesigning the magnetic step at every scale.
Why Conventional Magnets Create Variability
With a conventional magnetic rack or localized magnet, the force experienced by a bead changes according to its position in the vessel.
Close to the Magnet
Beads move rapidly and may become densely compacted. This increases the risk of clumping, impurity entrapment and difficult resuspension.
Farther from the Magnet
Beads move more slowly and may remain suspended when the supernatant is removed, reducing recovery.
When Volume Increases
The distance between the beads and the magnet changes. The magnetic conditions that worked in a small tube may therefore behave differently in a larger vessel.
How Sepmag Applies Constant Magnetic Force
Sepmag uses a proprietary cylindrical magnetic configuration around the vessel to create radial separation conditions.
Instead of pulling all the beads toward a single localized high-force point, the system applies a constant magnetic force throughout the working volume. Beads move toward the vessel wall under defined conditions, regardless of their starting position.
Because capture is distributed around the vessel, bead movement is more controlled and excessive local compaction is reduced.
From Defined Force to a Scalable Process
During scale-up, the distance that beads must travel may increase, so the separation time can change. The advantage of Sepmag technology is that the driving force remains constant and defined.
This makes separation behavior easier to characterize, transfer and validate instead of relying on trial-and-error adjustments at every new volume.
When combined with Sepmag real-time monitoring, the separation curve can also be used to define the endpoint, compare runs and identify changes in bead or process behavior.
Make the Magnetic Separation Step a Defined Process
Tell us your bead type, concentration, buffer conditions, working volume and vessel format. Sepmag will help you identify the appropriate system and establish a scalable separation strategy.
