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Gold has always held a special allure for humans throughout history: wars were fought over it, alchemists strived to turn other metals into it, graves were looted for it, and love was sworn by it. Gold is so entwined in our lives that many people don’t even realize that it is fundamental to many state-of-the-art biotechnology. One of the most useful modern properties of gold is the creation of a surface plasmon resonance condition upon exposure to incident light of a resonance frequency. Gold used in this way is applied as a thin layer on a surface plasmon resonance (SPR) chip or is used as nanoparticles in Surface Enhanced Raman Scattering (SERS). These aqueous solutions of gold nanoparticles (5-50 nm in diameter) are the least recognizable form of gold because these colloidal solutions actually appear reddish in color rather than the characteristic yellow color of larger solid gold. It turns out that the work of the alchemists was not wasted. The discovery of aqua regia (noble or royal water) by an alchemist in the 8th century AD is critical to gold nanoparticle synthesis. This powerful mixture of nitric acid and hydrochloric acid is capable of dissolving solid gold to make chloroauric acid, which is the starting point for gold nanoparticle synthesis.

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Uses for Gold Nanoparticles

Gold nanoparticles are gaining popularity as substrates for state-of-the-art sensing and imaging techniques. It is a straightforward process to attach oligonucleotides, antibodies, and other proteins to the surfaces of the gold nanoparticles. Gold nanoparticles are useful in biosensing because of that aformentioned property of surface plasmon resonance. A baseline resonance condition is established before any target molecule binds to the gold nanoparticles. Once the binding event occurs it is measured by a shift in the resonant wavelength. This sensing technique enables highly sensitive sensing instruments that can detect low levels of target molecule. Other sensing methods rely on changes in conductivity or redox behavior upon target binding.

Gold nanoparticles are commonly used in Surface Enhanced Raman Scattering (SERS) to enhance the raman signal. Certain vibrational patterns and raman shifts are indicative of certain bonds or functional groups in the molecule. The surface plasmon resonance from photons incident on the gold surface amplify these characteristic raman shifts.

Another use for gold nanoparticles is as a catalyst for a chemical reaction. This is particularly useful for alkyne activation reactions because gold acts as a Lewis acid. For the catalytic properties to be most useful, a uniform particle size is desired. The strength of the catalytic activity is inversely dependent on size. As the particles get smaller, there is an overall increase in surface area, which leads to greater catalytic activity.

Gold Nanoparticle Synthesis: reduction of chloroauric acid

Chloroauric acid (HAuCl4) contains the Au3+ ion. This ion is reduced to Au(0) by adding citrate (ex: trisodium citrate dihydrate) or sodium borohydride (NaBH4) in the presence of alkanethiols to stabilize the particles. The citrate is a convenience choice because it can both reduce and stabilize the particles. The concentration of reductant matters: Higher concentration leads to smaller particles overall because there are more seeding sites and more material to surround and stabilize the particles. Different concentrations of tween 80 have also been used to control particle size. After the particles are seeded they are allowed to ripen and grow slightly larger as small pieces of Au(0) join with larger conglomerates. This ripening process, similar to Ostwald ripening, can change a “knobby” particle to a smooth particle. If all parameters of the gold nanoparticle synthesis are performed carefully, then a fairly uniform batch of aqueous spherical nanoparticles will be produced and ready to use in  new experiments.

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Lluis M. Martínez | SEPMAG Chief Scientific Officer

Founder of SEPMAG, Lluis holds a PhD in Magnetic Materials by the UAB. He has conducted research at German and Spanish academic institutions. Having worked in companies in Ireland, USA and Spain, he has more than 20 years of experience applying magnetic materials and sensors to industrial products and processes. He has filed several international patents on the field and co-authored more than 20 scientific papers, most of them on the subject of magnetic particle movement.

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