Skip to main content
magnetic separation rack, scaling biomagnetic separation processes

4 usual Problems When Configuring a Large Volume Processing System

Working with magnetic separation rack? Keep reading!

Download our Free Guide on Biomagnetic Separation Scale-up HERE.

This post is about Magnetic Bead Separation with a magnetic separation rack, and how to scale-up this process. If you are interested in this topic, download our free eBook The Basic Guide to Scale-up Magnetic Bead Separation Processes:

Problems when scaling up

Normally, when companies want to scale up a process, they try to simply use bigger systems. If they do not try to analyze and define the process well, there are four typical problems that they will encounter when attempting to scale up a non-homogeneous Magnetic Bead Separation process.

  1. Larger batches will not have the same characteristics as smaller lots. Often larger batches will have different bead surface areas activated, more beads that are clumped, and the density of beads in the final volume is different.

  2. When you increase the volume without carefully defining the scale up process, you will have a greater loss of beads and biomolecules. Often you will find a 15-20% loss of material.

  3. In larger volumes irreversible aggregation can be a serious issue, even if it was not an issue in smaller volumes.

  4. Aliquots from the same batch for the individual IVD kits have characteristics that can vary much more than aliquots from smaller batches.

Therefore, it is important to understand the conditions of your process in order to avoid the above problems during scale-up. It is important to note that using a homogeneous Magnetic Bead Separation system will help you avoid the above problems since the parameters are well-defined.

Don’t forget to check these posts from our blog in order to get a deeper insight into the scaling-up of Magnteic Bead Separation processes:

Check to access to FREE eBooks on the subject, or contact us. We will be glad to help you to achieve an efficient magnetic bead separation process!

Dr. Lluís Martínez

magnetic separation rack

Lluis M. Martínez | SEPMAG Chief Scientific Officer

Founder of SEPMAG, Lluis holds a PhD in Magnetic Materials by the UAB. He has conducted research at German and Spanish academic institutions. Having worked in companies in Ireland, USA and Spain, he has more than 20 years of experience applying magnetic materials and sensors to industrial products and processes. He has filed several international patents on the field and co-authored more than 20 scientific papers, most of them on the subject of magnetic particle movement.

Leave a Reply